Stress-Activated Degradation of Sphingolipids Regulates Mitochondrial Function and Cell Death in Yeast

Figure 4

Yeast Ybh3p is not involved in hexadecenal-mediated cell death. (a) Mitochondrial association of Ybh3p is stimulated by acetic acid, but not by hexadecenal. Intracellular localization relative to the MitoTracker-stained mitochondria of a Ybh3-GFP fusion protein expressed in wild type was observed upon acetic acid (100 mM, 1 h at pH 3.0) or hexadecenal (50 μM, 1 h) treatment. (b) Sensitivity to hexadecenal is not altered in ybh3Δ mutants. The growth efficiency upon external addition of hexadecenal was assayed as in Figure 3(a) in wild type and ybh3Δ mutants.

(a)

(b)