Research Article

Stress-Activated Degradation of Sphingolipids Regulates Mitochondrial Function and Cell Death in Yeast

Table 1

Yeast strains used in this study.

NameRelevant genotypeSource

BY4741MATa; his3Δ1; leu2Δ0; met15Δ0; ura3Δ0EUROSCARF
BY4741 hfd1BY4741 with hfd1Δ::KanMXEUROSCARF
BY4741 faa1BY4741 with faa1Δ::KanMXEUROSCARF
BY4741 faa4BY4741 with faa4Δ::KanMXEUROSCARF
BY4741 dpl1BY4741 with dpl1Δ::KanMXEUROSCARF
BY4741 fzo1BY4741 with fzo1Δ::KanMXEUROSCARF
BY4741 dnm1BY4741 with dnm1Δ::KanMXEUROSCARF
BY4741 fis1BY4741 with fis1Δ::KanMXEUROSCARF
BY4741 hog1BY4741 with hog1Δ::KanMXEUROSCARF
BY4741 om14BY4741 with om14Δ::KanMXEUROSCARF
BY4741 mcr1BY4741 with mcr1Δ::KanMXEUROSCARF
BY4741 rdl1BY4741 with rdl1Δ::KanMXEUROSCARF
BY4741 mdm10BY4741 with mdm10Δ::KanMXEUROSCARF
BY4741 mdm12BY4741 with mdm12Δ::KanMXEUROSCARF
BY4741 ybh3BY4741 with ybh3Δ::KanMXEUROSCARF
BY4741 msn2msn4BY4741 with msn2Δ::Nat msn4Δ::KanMXE. de Nadal
BY4741 Hfd1-GFP Om14-dsRedBY4741 with plasmids pAG426-GPD-HFD1-GFP (URA3) + pAG415-GPD-OM14-dsRedThis study
BY4741 GAL1-ccdB-HABY4741 with plasmid pAG426-GAL1p-ccdB-HA (URA3)This study
BY4741 GAL1-DPL1-HABY4741 with plasmid pAG426-GAL1p-DPL1-HA (URA3)This study
hfd1 GAL1-ccdB-HABY4741 hfd1Δ::KanMX with plasmid pAG426-GAL1p-ccdB-HA (URA3)This study
hfd1 GAL1-DPL1-HABY4741 hfd1Δ::KanMX with plasmid pAG426-GAL1p-DPL1-HA (URA3)This study
W303-1AMATa; ade2-1; his3-11,-15; leu2,3-112; ura3-1; trp1R. Rothstein
W303-1A BaxW303-1A with plasmid pCM189-Bax (TetOff-Bax-c-myc, URA3)This study
W303-1A Bcl-xLW303-1A with plasmid pCM184-Bcl-xL (TetOff-Bcl-xL-c-myc, TRP1)This study
W303-1A pCM189W303-1A with plasmid pCM189This study
W303-1A pCM184W303-1A with plasmid pCM184This study
BY4741 BaxBY4741 with plasmid pCM189-Bax (TetOff-Bax-c-myc, URA3)This study
hfd1 BaxBY4741 hfd1Δ::KanMX with plasmid pCM189-Bax (TetOff-Bax-c-myc, URA3)This study
hog1 BaxBY4741 hog1Δ::KanMX with plasmid pCM189-Bax (TetOff-Bax-c-myc, URA3)This study
BY4741 pCM189BY4741 with plasmid pCM189This study
hfd1 pCM189BY4741 hfd1Δ::KanMX with plasmid pCM189This study
hog1 pCM189BY4741 hog1Δ::KanMX with plasmid pCM189This study
BY4741 Ybh3-GFPBY4741 with plasmid pUG36-GFP-YBH3 (URA3)This study
BY4741 Hfd1-GFP Mmm1-mCherryBY4741 with plasmids pAG426-GPD-HFD1-GFP (URA3) + pAG413-GAL1-MMM1-mCherry (HIS3)This study
mdm10 Hfd1-GFPBY4741 mdm10Δ::KanMX with plasmid pAG426-GPD-HFD1-GFP (URA3)This study
mdm12 Hfd1-GFPBY4741 mdm12Δ::KanMX with plasmid pAG426-GPD-HFD1-GFP (URA3)This study
mmm1 Hfd1-GFPBY4741 mmm1Δ::KanMX with plasmid pAG426-GPD-HFD1-GFP (URA3)This study
BY4741 mt-GFPBY4741 with plasmid pVT100U-mtGFP (URA3)B. Westermann
dnm1 mt-GFPBY4741 dnm1Δ::KanMX with plasmid pVT100U-mtGFP (URA3)This study
fis1 mt-GFPBY4741 fis1Δ::KanMX with plasmid pVT100U-mtGFP (URA3)This study