MicroRNA-93 Regulates Hypoxia-Induced Autophagy by Targeting ULK1

Figure 4

Re-expression of ULK1 without miR-93 response elements restores autophagy inhibited by miR-93. (a) MEFs were transfected with scramble NC (NC) and miR-93 mimics (93) or cotransfected miR-93 mimics (93) with the indicated constructs (ULK1-Myc) for 24 h by using the normal group as a control. Protein samples were collected and the expression of ULK1, Myc, LC3, and P62 was analyzed through Western blot. Actin was used as a loading control. (b) MEFs were fixed and immunostained by donkey anti-rabbit 488 or donkey anti-mouse 555. Scale bar, 20 μm. (c) MEFs were treated the same as (a). Cells were fixed and immunostained by anti-ULK1 (green) and anti-P62 (red) antibodies. Scale bar, 20 μm.

(a)

(b)