MicroRNA-93 Regulates Hypoxia-Induced Autophagy by Targeting ULK1

Figure 5

The effects of miR-93 on cell viability and apoptosis in noncancer cell lines and cancer cells. (a, b) CCK8 proliferation assays were performed on MEFs transfected with indicated RNA for 12 h. MEFs were exposed to starvation or hypoxic conditions for another 24 h. The graph represents the cell proliferation of three replicates with standard deviation represented by error bars. ; . (c) MEFs were transfected with the indicated microRNAs or left untransfected for 12 h; the cells were exposed to hypoxic condition for another 24 h. The cell apoptosis rate was detected by flow cytometry. Cellular apoptosis rate was calculated by GraphPad Prism 5. (d) HeLa cells were transfected with scramble NC (NC), miR-93 mimics (93), scramble IN-NC (IN-NC), or miR-93 inhibitor (IN-93) for 12 h, followed by hypoxic treatment for another 24 h. Normal group was set as control. The CCK8 proliferation assays were detected. ; . (e) HeLa cells were treated as (d), then subjected to TUNEL staining. DAPI (blue), TUNEL-positive cells (green). Magnification, 20x.

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