Cytotoxicity, Oxidative Stress, Cell Cycle Arrest, and Mitochondrial Apoptosis after Combined Treatment of Hepatocarcinoma Cells with Maleic Anhydride Derivatives and Quercetin

Figure 4

Effect of the treatments on ROS production assessed from the oxidation of DCFDA by hydrogen peroxide using fluorometric assay on HuH7 and HepG2 cells at 24 hours posttreatment; (b) determination of GSH and GSSG levels by fluorometric assay per mmol/mg protein at 24 hours posttreatment in HuH7 and HepG2 cells. All data are presented as the mean ± SEM of 3 experiments; statistical evaluations were performed using Tukey’s one-way ANOVA to obtain significant differences (, , and ) with normalization based on the vehicle group treated with DMSO.

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