Cytotoxicity, Oxidative Stress, Cell Cycle Arrest, and Mitochondrial Apoptosis after Combined Treatment of Hepatocarcinoma Cells with Maleic Anhydride Derivatives and Quercetin

Figure 7

Quantification of pyknotic nuclei by staining with Hoechst in cells (a), (c) HuH7 cells, (b) (d) HepG2 cells, and (e), (f) HaCaT cells at 24 hours and 48 hours posttreatment. All data are presented as the mean ± SEM of 3 experiments; statistical evaluations were performed using Tukey’s one-way ANOVA to obtain significant differences (, ) with normalization based on the vehicle group treated with DMSO. (g) TUNEL assay in HepG2 cells; nuclear staining of Hoechst is shown in red, and TUNEL is shown in green at a magnification of 40x.

(a)

(b)

(c)

(d)

(e)

(f)

(g)