Cytotoxicity, Oxidative Stress, Cell Cycle Arrest, and Mitochondrial Apoptosis after Combined Treatment of Hepatocarcinoma Cells with Maleic Anhydride Derivatives and Quercetin

Figure 8

Activation of the intrinsic pathway of apoptosis after the effect induced by individual and combination treatments. Expression of procaspase-9 at 46 kDa and activation of caspase-9 at 35 kDa in (a) HuH7 and (b) HepG2 cells at 24 h posttreatment. Expression of procaspase-3 at 32 kDa and activation of caspase-3 at 21 kDa were observed at 36 hours posttreatment in (g) HuH7 and (h) HepG2 cells. For all cases, actin was used as a control of protein loading. (c), (d), (e), (f), (i), and (j) graphs corresponding to the colorimetric quantification by ImageJ. All data are presented as the mean ± SEM of 4 experiments; statistical evaluations were performed using one-way ANOVA with Tukey’s test to obtain significant differences (, , and ) with normalization based on the vehicle group treated with DMSO.

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