Oleate Prevents Palmitate-Induced Atrophy via Modulation of Mitochondrial ROS Production in Skeletal Myotubes

Figure 4

The effects of oleic acid and MitoTEMPO on mitochondrial biogenesis and dynamics in PA-treated myotubes. (a) Differentiated myotubes were cotreated with 0.3 mM palmitate and different doses of oleate (0.1, 0.3, 0.5, and 1 mM) for 30 h. The myotubes were visualized by florescence microscope after MitoTracker staining. (b) Florescence intensity of MitoTracker was quantified. (c) Gene expressions of mitochondrial fission, (d) fusion, and (e) biogenesis were quantified. Differentiated myotubes were cotreated with 0.3 mM palmitate and/or 0.5 mM oleate for 30 h. For some cells, 25 nM of MitoTEMPO was treated 1 h prior to palmitate treatment. Bar graph represented means ± SE. Scale bar = 100 μm. The data were analyzed using one-way ANOVA followed by a Tukey’s post hoc test. versus NT. NT: nontreatment; PA: palmitic acid; PA + OA: palmitic acid with oleic acid; PA + MitoT: palmitic acid + MitoTEMPO.

(a)

(b)

(c)

(d)

(e)