Aspalatone Prevents VEGF-Induced Lipid Peroxidation, Migration, Tube Formation, and Dysfunction of Human Aortic Endothelial Cells

Figure 2

Aspalatone inhibits VEGF-induced migration of HAECs. (a) In vitro wound healing assay showing effect of aspalatone on VEGF-induced migration in HAECs. HAECs were grown in 12-well tissue culture dishes and after the cells became confluent, the monolayer was scratched with a sterile pipette tip. The media was replaced with VEGF (10 ng/mL) without or with aspalatone (50 μM) and the cells were incubated for the indicated time periods. Photographs were taken at 0 h and 18 h when the VEGF containing monolayer wound was completely filled. (b) Histogram showing migration rate calculated by formula (−)/. Representative photographs from three independent experiments are shown. Magnification 10x. Scale bar: 400 μm.

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