Aspalatone Prevents VEGF-Induced Lipid Peroxidation, Migration, Tube Formation, and Dysfunction of Human Aortic Endothelial Cells

Figure 4

Aspalatone inhibits VEGF-induced adhesion of monocytes to HAECs. (a) HAECs (3000 cells/well) in 96-well plates were treated with aspalatone (50 μM) overnight followed by addition of THP-1 cells and VEGF (10 ng/mL) for another 18 h. Cell adhesion was determined by MTT absorbance recorded at 570 nm using a plate reader. Bars represent mean ± SD (). when compared with control and when compared with VEGF-treated group. HAECs treated without or with (b) VEGF (10 ng/mL) or (c) LPS (1 μg/mL) in the absence and presence of aspalatone (50 μM) for 18 h. Equal amounts of cell extracts were subjected to Western blot analysis using specific antibodies against VCAM-1, ICAM-1, eNOS, iNOS, and GAPDH in HAECs.

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