Basic Fibroblast Growth Factor Inhibits Apoptosis and Promotes Proliferation of Adipose-Derived Mesenchymal Stromal Cells Isolated from Patients with Type 2 Diabetes by Reducing Cellular Oxidative Stress

Figure 3

The effect of bFGF stimulation on ASCs’ proliferative activity and clonogenic potential. Growth kinetics of diabetic-ASCs after bFGF treatment at concentrations of 5 ng/mL and 10 ng/mL in comparison to nontreated healthy and diabetic control ASCs (a). Supplementation of culture medium with bFGF resulted in restoration of reduced proliferation rate of diabetic-ASCs to the level of healthy-ASCs after 120 h of propagation. Population doubling time calculated after 120 h of cell propagation (b). bFGF-treated diabetic-ASCs were characterized by significantly abbreviated time required to double the population. CFU-E assay showing impaired clonogenic potential of diabetic-ASCs when compared to healthy-ASCs (c). Exposition to bFGF caused increase in the number of clonogenic precursors in diabetic-ASC cultures. Identification of proliferating cells by immunocytochemical staining for Ki-67 (d). Quantification of Ki-67 staining showing decreased percentage of Ki-67 expressing cells among diabetic-ASCs in respect to healthy-ASCs and increment in the number of proliferating cells following bFGF stimulation; fraction of Ki-67 positive cells was calculated on the basis of image analysis of 10 randomly selected pictures (e). Results are expressed as mean ± SD. value <0.05, p value <0.01, and value <0.001.

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