Basic Fibroblast Growth Factor Inhibits Apoptosis and Promotes Proliferation of Adipose-Derived Mesenchymal Stromal Cells Isolated from Patients with Type 2 Diabetes by Reducing Cellular Oxidative Stress
Table 1
Sequences of primers used in real-time RT-PCR.
Gene
Sequence 5′-3′
Amplicon length (bp)
Accession number
p21
F: AGAAGAGGCTGGTGGCTATTT
169
NM_001220777.1
R: CCCGCCATTAGCGCATCAC
p53
F: AGATAGCGATGGTCTGGC
381
NM_001126118.1
R: TTGGGCAGTGCTCGCTTAGT
Bcl-2
F: ATCGCCCTGTGGATGACTGAG
129
NM_000633.2
R: CAGCCAGGAGAAATCAAACAGAGG
Bax
F: ACCAAGAAGCTGAGCGAGTGTC
365
XM_011527191.1
R: ACAAAGATGGTCACGGTCTGCC
SIRT1
F: ACAGGTTGCGGGAATCCAAA
155
NM_001314049.1
R: GTTCATCAGCTGGGCACCTA
FGFR-1
F: CCGCCCAACTTTTCCTCCAA
511
XM_006716314.1
R: AGGTGGCATAACGGACCTTG
GLUT-4
F: CTCTGAGTTGAGGGCAAGGG
3734
XM_011523750.1
R: GGGGGACCTACCGCAACATA
TET2
F: GAGACGCTGAGGAAATACGG
258
NM_001127208.2
R: TGGTGCCATAGGAGTGGACA
TET3
F: CAGACCGCTGTGATCGTCAT
263
NM_001287491.1
R: AACTTGCGAGGTGTCTTGCT
GAPDH
F: GTCAGTGGTGGACCTGACCT
256
NM_001289746.1
R: CACCACCCTGTTGCTGTAGC
F: sense primer; R: antisense primer; bp: base pair.
