Figure 2: Sample images of a JC-1-labeled hippocampal astrocyte. Displayed are the individual raw images acquired by the two detection channels, representing JC-1 monomers and J-aggregates, respectively. The offline computed overlay image confirms the perfect alignment of the spectrally differing images. The calculated JC-1 ratio (red/green) represents the ΔΨm range of individual mitochondria and their morphological/functional heterogeneity.