Increased Mitochondrial Mass and Cytosolic Redox Imbalance in Hippocampal Astrocytes of a Mouse Model of Rett Syndrome: Subcellular Changes Revealed by Ratiometric Imaging of JC-1 and roGFP1 Fluorescence

Figure 6

Optical redox imaging with the genetically encoded roGFP1 sensor confirms more oxidized conditions in Mecp2^−/y astrocytes. (a) CCD camera image of a Mecp2^−/y astrocyte expressing cytosolic roGFP1. (b) Under control conditions, fluorescence ratios were higher (more oxidized) in the cytosol of Mecp2^−/y than of WT astrocytes. Trolox induced a shift towards more reducing conditions especially in Mecp2^−/y astrocytes (, ^#). (c) Calculating the relative degree of roGFP1 oxidation (OxD_roGFP1) and the corresponding reduction potentials (E_roGFP1) confirms the more oxidized conditions in Mecp2^−/y astrocytes as well as the antioxidant effect mediated by Trolox (^###).

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