Increased Mitochondrial Mass and Cytosolic Redox Imbalance in Hippocampal Astrocytes of a Mouse Model of Rett Syndrome: Subcellular Changes Revealed by Ratiometric Imaging of JC-1 and roGFP1 Fluorescence

Figure 7

WT and Mecp2^−/y astrocytes show similar vulnerabilities to mitochondrial compromise. (a) CN⁻ treatment (100 μm, 5 min) evoked a clear decrease in JC-1 ratio, which is evident as the green shift of the dual color emission and indicates a marked mitochondrial depolarization; displayed is a WT astrocyte. (b) Significant changes in mitochondrial content (mitochondria/cell) were not evoked by FCCP or CN⁻ treatment. (c) The length of individual mitochondria was affected only very moderately (^##, ^###). (d) As expected, both treatments evoked a clear mitochondrial depolarization. Significant genotypic differences were not observed in response to chemically induced hypoxia (CN⁻) or mitochondrial uncoupling (FCCP).

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