(a)
(b)
(c)
(d)
Figure 7: WT and Mecp2−/y astrocytes show similar vulnerabilities to mitochondrial compromise. (a) CN treatment (100 μm, 5 min) evoked a clear decrease in JC-1 ratio, which is evident as the green shift of the dual color emission and indicates a marked mitochondrial depolarization; displayed is a WT astrocyte. (b) Significant changes in mitochondrial content (mitochondria/cell) were not evoked by FCCP or CN treatment. (c) The length of individual mitochondria was affected only very moderately (##, ###). (d) As expected, both treatments evoked a clear mitochondrial depolarization. Significant genotypic differences were not observed in response to chemically induced hypoxia (CN) or mitochondrial uncoupling (FCCP).