EGCG Prevents High Fat Diet-Induced Changes in Gut Microbiota, Decreases of DNA Strand Breaks, and Changes in Expression and DNA Methylation of Dnmt1 and MLH1 in C57BL/6J Male Mice

Table 2

Sequence to analyze primers for CpG methylation analysis.

Gene	Primer	Sequence (5′-3′)	Size (bp)	GC%
Dnmt1	FW	Biotin - GTA GGT TGT AGA AGA TAG AAT AGT TTT GA	29	31
	RW	CCC ACT CTC TTA CCC TAT ATA ATA CAT	27	37
	Seq	CCC CTC CCA ATT AAT TTC	18	44.4
	Sequence ID: gb|AH009208.2|
	Dnmt1: at reverse strand of chromosome 9: 20907205–20959888 (52684 bp).
Sequence to analyze	7104 – CGCGCGCGCGAAAAAGCCGGGGTCTCGT - 7131		27	7 CpGs
MLH1	FW	AGG GTA TTT TAG TTT TTA TTG GTT GGA GA	29	31
	RW	TTA CAC CTC AAT TCC TAA AAT CTC TAT CCC – Biotin	30	37
	Seq	TTT AGT TTT TAG AAA TGA GTT AAT A	25	16
	Sequence ID: ref|XR_379849.3|
	MLH1: at reverse strand of chromosome 9: 111228228–111271786 (43559 bp)
Sequence to analyze	19 - GAAGAGCGGACCGTGAACTTTGACGCGCAAGCGCG		64	8 CpGs
	TTGCCTTCTA-GCCTGGTGTCGGGCCGCTG - 82