EGCG Prevents High Fat Diet-Induced Changes in Gut Microbiota, Decreases of DNA Strand Breaks, and Changes in Expression and DNA Methylation of Dnmt1 and MLH1 in C57BL/6J Male Mice

Table 3

Primers and TaqMan®-Probes targeting 16rRNA coding regions of bacteria.

Target organism	Primer/probe	Sequence (5′-3′)	Size (bp)	Conc. [pmol/µL]	Reference
All bacteria	Fwd primer	ACT CCT ACG GGA GGC AG	468	10	[15]
	Rev primer	GAC TAC CAG GGT ATC TAA TCC		10
	Probe	(6-FAM)-TGC CAG CAG CCG CGG TAA TAC-(BHQ-1)		2
Clostridium cluster IV (Ruminococcaceae)	Fwd primer	GCA CAA GCA GTG GAG T	239	4	[16]
	Rev primer	CTT CCT CCG TTT TGT CAA		4
	Probe	(6-FAM)-AGG GTT GCG CTC GTT-(BHQ-1)		2
Cluster XIVa (Lachnospiraceae)	Fwd primer	GCA GTG GGG AAT ATT GCA	477	5	[16]
	Rev primer	CTT TGA GTT TCA TTC TTG CGA A		5
	Probe	(6-FAM)-AAA TGA CGG TAC CTG ACT AA-(BHQ-1)		1,5
Bacteroidetes	Fwd primer	GAG AGG AAG GTC CCC CAC	106	3	[17]
	Rev primer	CGC TAC TTG GCT GGT TCA G		3
	Probe	(6-FAM)-CCA TTG ACC AAT ATT CCT CAC TGC TGC CT-(BHQ-1)		1
Bifidobacterium spp.	Fwd primer	GCG TGC TTA ACA CAT GCA AGT C	125	3	[18]
	Rev primer	CAC CCG TTT CCA GGA GCT ATT		3
	Probe	(6-FAM)-TCA CGC ATT ACT CAC CCG TTC GCC-(BHQ-1)		1.5