A Cystine-Rich Whey Supplement (Immunocal®) Provides Neuroprotection from Diverse Oxidative Stress-Inducing Agents In Vitro by Preserving Cellular Glutathione

Figure 4

Immunocal preserves CGN viability and protects from apoptosis after exposure to SNP. (a) Representative images of CGNs left untreated (control), treated with SNP (100 μM), or preincubated with Immunocal for 24 h before SNP treatment for further 24 h. Immunofluorescence shows β-tubulin (green) and DAPI (blue). Scale bar, 10 μm. (b) Quantification of apoptosis for 5 independent experiments performed as in (a). Results are shown as mean ± SEM, . (c) MTT cell viability was measured as described in Materials and Methods. Results are shown as mean ± SEM, . For (b) and (c), indicates compared to control, and ††† indicates compared to SNP. Con: control; ICAL: Immunocal.

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