Research Article
Dietary Flaxseed Oil Prevents Western-Type Diet-Induced Nonalcoholic Fatty Liver Disease in Apolipoprotein-E Knockout Mice
Figure 10
Supplementation of flaxseed oil decreased mRNA and protein expressions of hepatic inflammatory cytokines in mice. Quantitative real-time RT-PCR and Western blot analysis of the mRNA and protein expressions of (a) IL-6, (b) TNF-α, and (c) MCP-1 in the liver of mice in each group. Fold changes of mRNA levels were determined after normalization to internal control β-actin RNA levels. Blotting with anti-β-actin was used as a protein loading control. Protein expressions were presented as fold change relative to control. Representative immunoblots are shown. Each bar denotes mean ± SEM ( mice). A versus the control group; B versus the WTD group.
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