Diosmin Attenuates Methotrexate-Induced Hepatic, Renal, and Cardiac Injury: A Biochemical and Histopathological Study in Mice

Table 1

Ameliorative effects of diosmin on serum concentrations of hepatic, cardiac, and renal injury markers in mice exposed to methotrexate.

Group	AST	ALT	ALP	LDH	CK	CK-MB	Urea	Creatinine
	(U/ml)	(U/ml)	(U/l)	(U/l)	(U/l)	(U/l)	(mg/dl)	(mg/dl)
Control	30.7 ± 1.8	27.2 ± 1.3	68.3 ± 1.6	290.5 ± 7.3	116 ± 3.1	36.6 ± 1.3	22.3 ± 1.5	0.41 ± 0.02
MTX	83 ± 4.6	71.5 ± 2.7	132.7 ± 3.03	440.7 ± 10.3	394 ± 17.6	190.3 ± 15.6	73.5 ± 3.5	2.1 ± 0.15
MTX-DM50	52 ± 4.7^#	42 ± 2^#	84.6 ± 2.5^#	347.2 ± 15.7^#	221 ± 11.6^#	89.4 ± 4.3^#	39 ± 2.3^#	1.2 ± 0.13^#
MTX-DM100	36.9 ± 2.8^#	30 ± 1.4^#	71.2 ± 2.6^#	307.6 ± 9^#	146 ± 9.7^#	45.7 ± 4.3^#	26.4 ± 2.4^#	0.57 ± 0.04^#

Values are means ± SEM. Significant change at with respect to the negative control group. ^#Significant change at with respect to the MTX group as the positive control group. ALP: alkaline phosphatase; ALT: alanine transferase; AST: aspartate transferase; CK: creatine kinase; LDH: lactate dehydrogenase.