Genetic Nrf2 Overactivation Inhibits the Deleterious Effects Induced by Hepatocyte-Specific c-met Deletion during the Progression of NASH

Figure 1

Genotyping of c-met gene performed on cDNA transcribed from hepatic RNA showing amplification of the floxed region of the gene with and without cre-recombinase activation ((a), upper panel). Western blot panel for Keap1 and Nrf2 protein expression analysis ((a), lower panels). Representative pictures of hematoxylin and eosin (H&E) staining on liver section of c-met^fx/fx, c-met^Δhepa, and met/Keap1^Δhepa mice after 4 weeks chow (left panels) and MCD (right panels) diet (b). Weekly growth curve of c-met^fx/fx, c-met^Δhepa, and met/Keap1^Δhepa mice during a 4-week course of MCD treatment. Data are expressed as mean ± SE, Student’s t-test with () (c). Liver body weight ratio (d) and intrahepatic triglyceride content measurement (e) after 4-week chow and MCD diet feeding. Data are expressed as mean ± SE, ANOVA test with ().

(a)

(b)

(c)

(d)

(e)