Triethylenetetramine Synergizes with Pharmacologic Ascorbic Acid in Hydrogen Peroxide Mediated Selective Toxicity to Breast Cancer Cell

Figure 6

The effects of SOD on Asc/TETA induces apoptosis. (a) 1 mM Asc, 10 μM TETA, 100 U/mL SOD1, 1 mM Asc/10 μM TETA, or 1 mM Asc/10 μM TETA plus SOD was applied onto MCF-7 cells for 12 hours. Cloning formation assay was performed to determine the plating efficiency. , , ; (b) SOD1 expression levels in HS578Bst and MCF-7 cells were assessed by western blotting; (c) downregulation of SOD1 expression in MCF-7 cells by SOD1 shRNA was determined by western blotting; (d) MCF-7 cell viability after SOD1 shRNA treatment was measured by MTT assay, , .

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