Oxidative Medicine and Cellular Longevity / 2017 / Article / Fig 6

Research Article

Hepatoprotective Effect of Polyphenol-Enriched Fraction from Folium Microcos on Oxidative Stress and Apoptosis in Acetaminophen-Induced Liver Injury in Mice

Figure 6

Effects of FMF on APAP-induced cell death-related gene expression. (a) Total cellular protein from liver tissues was extracted, and protein levels of CYP2E1, cleaved caspase-3/9, caspase-3/9, Bax, Bcl2, p-JNK, JNK, p-ERK, and ERK were determined by western blot. GAPDH was used as an endogenous control. (b) Total RNA from liver tissues was isolated and reverse-transcribed into cDNA for quantitative real-time PCR analysis of Bax and Bcl2 mRNA levels. GAPDH was used as an endogenous control. Results are shown as (). , compared with the control group; ### compared with APAP-intoxicated group.

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