Glycosides from Stevia rebaudiana Bertoni Possess Insulin-Mimetic and Antioxidant Activities in Rat Cardiac Fibroblasts

Figure 4

Effect of R97, R60, or insulin on Glut4 translocation to the plasma membrane in rat cardiac fibroblasts in the presence or absence of the antagonist of the insulin receptor S961. Cells were treated with R97 or R60 (1 mg/mL) for 1 hour or with insulin (100 nM) for 30 min in the presence or absence of 10 nM S961 for 90 min. Glut4 translocation to the plasma membrane was detected using immunofluorescence staining with anti-Glut4 antibody and DAPI nuclear staining as a counterstain. Images were acquired by the Nikon A1 confocal laser scanning microscope (Nikon Instruments, Japan). The results are representative of two independent experiments.