Glycosides from Stevia rebaudiana Bertoni Possess Insulin-Mimetic and Antioxidant Activities in Rat Cardiac Fibroblasts

Figure 6

Effects of different steviol glycoside mixtures or insulin on AMPK phosphorylation level in rat cardiac fibroblasts. (a) Rat cardiac fibroblasts treated with R97, R60, SG, or TRU (1 mg/mL) for 1 hour or with insulin (100 nM) for 30 min were lysed with RIPA buffer. Cell lysates were electrophoresed and immunoblotted with anti-AMPK and anti-phospho-AMPK, as described in the experimental procedure section. Actin detection was used as a control. Immunoblots are representative of three independent experiments. (b) Densitometric analysis of AMPK phosphorylation level is expressed as phospho-AMPK/total AMPK and reported as folds to control.

(a)

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