Resveratrol Attenuates Copper-Induced Senescence by Improving Cellular Proteostasis

Figure 1

Reduced sirtuin 1 expression in CuSO₄-SIPS fibroblasts is restored by the addition of resveratrol. (a-b) WI-38 fibroblasts were incubated with 350 µM CuSO₄ (or Na₂SO₄, for controls) for 24 h. Then, media were changed and cells were allowed to recover for an additional 72 h period in the presence of 5 or 10 µM resveratrol (or 0.1% DMSO, for controls). After this recovery period, fibroblasts were processed for different assays. (a) Sirtuin 1 (Sirt1) transcript levels were assessed by qPCR and plotted assuming that mRNA level of controls equals 1. TBP was the selected housekeeping gene. (b) Sirt1 relative protein content was determined by western blot, using Ponceau S staining to normalize protein loading. Depicted blots are representative and densitometric quantification is plotted assuming that control cells in the absence of resveratrol presents a relative protein level of 1. Data represent mean ± SEM of at least three independent experiments. and , when compared to control cells in the absence of resveratrol; , relatively to CuSO₄-treated cells without resveratrol.

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