Sulforaphane Protects against High Cholesterol-Induced Mitochondrial Bioenergetics Impairments, Inflammation, and Oxidative Stress and Preserves Pancreatic β-Cells Function

Figure 3

SFN induces the expression of genes regulating mitochondrial function. Gene expression of (a) Sirt1 and (b) Pgc-1α in Min6 cells treated with 320 µM of cholesterol and/or 10 µM of SFN or ES for 20 h was evaluated. Values are expressed as relative fold expression of two housekeeping genes (Table 2). Values are expressed as mean ± SEM, from three independent culture preparations, each treatment performed in quadruplicate. All two-ways ANOVAs and Bonferroni post-tests. Values with different superscript letters indicate significant differences between groups. Chol: cholesterol; SFN: sulforaphane; ES: 3,4-dihydroxyphenylacetic acid; Sirt1: sirtuin 1; and Pgc-1α: peroxisome proliferator-activated receptor gamma coactivator-1-alpha.

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