Sulforaphane Protects against High Cholesterol-Induced Mitochondrial Bioenergetics Impairments, Inflammation, and Oxidative Stress and Preserves Pancreatic β-Cells Function

Figure 5

SFN inhibits the increase in nuclear NFκB translocation in Min6 cells exposed to cholesterol. NFκB (p65) binding to its cognate sequence was quantified in nuclear extracts from cells incubated for 6 h with 320 µM of cholesterol and/or 10 µM of SFN or ES. Values are expressed as percentage relative to positive control (HeLa cell lysate containing TNFα-activated NFκB (p65), 100%). Values are expressed as mean ± SEM, from three independent culture preparations, each treatment performed in triplicate. All two-ways ANOVAs and Bonferroni post-tests. Values with different superscript letters indicate significant differences between groups. Chol: cholesterol; SFN: sulforaphane; and ES: 3,4-dihydroxyphenylacetic acid.