Sulforaphane Protects against High Cholesterol-Induced Mitochondrial Bioenergetics Impairments, Inflammation, and Oxidative Stress and Preserves Pancreatic β-Cells Function

Figure 6

SFN protects against impaired glucose-stimulated insulin secretion in Min6 cells exposed to cholesterol. Insulin levels in the media of Min6 cells were determined in the (a) absence of glucose, (b) in response to 1 h exposure to low glucose (5 mM), or (c) high glucose (25 mM) after 6 h preincubation with 320 µM of cholesterol and/or 10 µM of SFN or ES, from three independent culture preparations, each treatment performed in triplicate. Values are expressed as mean ± SEM. N = 3. All two-ways ANOVAs and Bonferroni post-tests. Values with different superscript letters indicate significant differences between groups. Chol: cholesterol; SFN: sulforaphane; and ES: 3,4-dihydroxyphenylacetic acid.

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(b)

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