Sulforaphane Protects against High Cholesterol-Induced Mitochondrial Bioenergetics Impairments, Inflammation, and Oxidative Stress and Preserves Pancreatic β-Cells Function

Table 1

Sulforaphane and ES prevent the changes in cytokines levels induced by cholesterol in Min6 cells.

	pg cytokines/mg protein
	IL-1β	TNFα	IFNγ	IL-4	IL-10
Control	74.15 ± 0.43	15.03 ± 0.40	31.22 ± 0.57	14.33 ± 0.59	85.20 ± 1.49
Chol 320 µM	82.02 ± 0.55	18.23 ± 1.85	36.66 ± 1.24	11.42 ± 1.10	75.13 ± 1.49
SFN 10 µM	69.90 ± 0.87	12.85 ± 0.25	27.51 ± 0.26	18.40 ± 0.58	89.00 ± 1.31
SFN 10 µM + chol 320 µM	65.25 ± 1.30	12.57 ± 0.35	29.23 ± 1.20	20.00 ± 0.22	85.32 ± 1.11
ES 10 µM	71.78 ± 1.16	11.86 ± 0.44	28.56 ± 0.34	16.39 ± 0.68	90.60 ± 1.64
ES 10 µM + chol 320 µM	71.28 ± 1.92	12.56 ± 0.38	28.50 ± 1.66	17.89 ± 0.39	86.25 ± 1.34

Proinflammatory and anti-inflammatory cytokines in Min6 cells treated for 20 h with 320 µM cholesterol and/or 10 µM SFN or ES. Values are expressed as mean ± SEM, from three independent culture preparations, each treatment performed in quadruplicate. All two-way ANOVA and Bonferroni posttests. Values with different superscript letters indicate significant differences between groups.