Tocotrienol-Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence-Associated Oxidative Stress in Human Myoblasts

Figure 3

Effects of antioxidants treatment on lipid peroxidation levels of myoblasts. (a) Young, (b) presenescent, and (c) senescent myoblasts labeled with C11-BODIPY® (581/591) to detect lipid peroxidation which were observed under fluorescence microscope (magnification: 200x, scale bar, 200 μm). TRF and ATF treatment effectively reduced lipid peroxidation. (d) Representative histogram of myoblasts based on the oxidized BODIPY (in green, 525/40 bandpass channel). (e) Ratio of oxidized/reduced BODIPY virtually representing lipid peroxidation in myoblast cells. Denoting , significantly different compared to young control; , significantly different compared to presenescent myoblasts; , significantly different compared to senescent myoblasts. Data are presented as the mean ± SD, .

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