Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils

Figure 3

Apoptosis rate of PMNs was detected by flow cytometry (). We used fMLP (10 nM) to stimulate PMNs for 2 min, the cells without fMLP as control. CaSR inhibitor NPS-2143 (100 nM), CaSR agonist cinacalcet (100 nM), and NF-κB inhibitor PDTC (100 nM) were added in cultured PMNs at 37°C for 30 min, and PMNs’ apoptosis rate was detected. Results were representative of three experiments (a). The apoptosis rate under different conditions was quantified by densitometry (b). ^# versus control group, versus fMLP group, and versus fMLP + CIN group.

(a)

(b)