Oxidative Medicine and Cellular Longevity / 2017 / Article / Fig 3

Research Article

circRNA_0046367 Prevents Hepatoxicity of Lipid Peroxidation: An Inhibitory Role against Hepatic Steatosis

Figure 3

circRNA_0046367 normalization abolishes the miR-34a-induced inhibition of PPARα. (a) Complementation between “seed sequence” of miR-34a and 3-UTR of PPARα predicts a miR-34a/PPARα interaction. (b) Dual luciferase reporter assay exhibits the inhibitory effect of miR-34a on PPARα. (c) circRNA_0046367 normalization restores the mRNA level of PPARα by miR-34a inactivation. (d), (e) Western blot (d) with quantification against GAPDH (e) shows the significant upregulation of PPARα in groups with normalized circRNA_0046367. The presented results are expressed as means ± S.D. .

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