Sirt1 Protects Endothelial Cells against LPS-Induced Barrier Dysfunction

Figure 2

Protein expression of Sirt1 and activity changes in HUVECs. (a) HUVECs were exposed to 500 ng/mL LPS for 1, 2, 4, 8, 12, and 24 h, and protein expression of Sirt1 was detected by WB. 0 h was used as the control. (b) Cells were stimulated with or without 500 ng/mL LPS for 1 h in the presence or absence of the RAGE antibody (10 μg/mL, 1 h). The immunoprecipitates were analyzed with anti-Sirt1 and anti-ubiquitin antibodies. (c) Sirt1 activity was examined and SRT1720 enhanced Sirt1 activity, while ex527 inhibited Sirt1 activity. (d) ECs were treated with either control siRNA or Sirt1 siRNA targeting Sirt1. The effect of siRNA on the Sirt1 protein expression was measured by WB. per group. versus control or control siRNA.

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