Sirt1 Protects Endothelial Cells against LPS-Induced Barrier Dysfunction

Figure 5

Involvement of RAGE and TLR4 in the LPS-induced Sirt1 decrease in ECs. (a) Pretreatment of the RAGE antibody (10 μg/mL) for 1 h attenuated Sirt1 decreases in HUVECs. (b) Knockout of RAGE attenuated the LPS-induced Sirt1 decrease in PMVECs. PMVECs of knockout mice and wild-type mice were incubated with or without LPS. Expression of Sirt1 was evaluated by WB. (c) Pretreatment of the RAGE antibody (10 μg/mL) and TLR4 inhibitor (3 μM) for 1 h attenuated the LPS-induced decrease in Sirt1 activity in HUVECs. . versus control or wild type without LPS treatment, ^# versus LPS or wild type with LPS treatment.

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