Reactive Oxygen Species Mediated Prostaglandin E₂ Contributes to Acute Response of Epithelial Injury

Figure 1

Rapid formation of ROS induced by scratching injury in HaCat cells. (a) The intracellular ROS were visualized by the reaction with probe carboxy-H₂DCFDA under fluorescent microscopy. Cells without damage worked as negative control, while H₂O₂ treated cells were used as positive control. (b) The quantities of ROS were shown after scratching for undamaged control cells, damaged cells, and damaged cells plus DPI treatment. Data are representative of three independent experiments with triplicate samples. and # indicate compared with undamaged control cells and with scratched cells, respectively.

(a)

(b)