Reactive Oxygen Species Mediated Prostaglandin E₂ Contributes to Acute Response of Epithelial Injury

Figure 3

Participation of ERK activation in scratching injury-induced release of ROS and PGE₂. (a) The change of p-ERK and ERK in HaCat cells treated with scratching injury for different periods of time as indicated. The protein levels of p-ERK and ERK were determined by immunoblot analysis and normalized to β-tubulin. (b) The level of p-ERK was decreased in HaCat cells preincubated with different concentrations of DPI for 30 min followed by scratches for 0.5 hr. (c) The dependence of PGE₂ production caused by scratching injury on ERK activation. Values are means ± SEMs of three replicates. and # indicate compared with undamaged control cells and with scratched cells, respectively.

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