Reactive Oxygen Species Mediated Prostaglandin E₂ Contributes to Acute Response of Epithelial Injury

Figure 4

Injury-induced PGE₂ release mediated by COX-2, not COX-1. (a) COX-1 and COX-2 activity of uninjured or injured HaCat cells were measured by COX activity assay. (b) COX-2 expression was increased in HaCat cells treated with scratching injury for different periods of time as indicated. The protein levels of COX-2 were determined by immunoblot analysis and normalized to β-tubulin. (c) The induced expression of COX-2 in HaCat cells was suppressed by NOX/ROS inhibitor DPI. (d) The induced expression of COX-2 in HaCat cells was suppressed by ERK inhibitor. (e) The induced expression of COX-2 in HaCat cells was suppressed by a COX-2 inhibitor. Values are means ± SEMs of three replicates. and # indicate compared with undamaged control cells and with scratched cells, respectively.

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