Amifostine Pretreatment Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Apoptosis and Oxidative Stress

Figure 9

Amifostine pretreatment reduces reactive oxygen species in TBHP-induced H9c2 cells and enhances the expression of SOD2. (a) Typical images of different TBHP concentrations on reactive oxygen species in H9c2 cells. 1: control; 2: 50 μM TBHP; 3: 100 μM TBHP; 4: 200 μM TBHP; 5: 300 μM TBHP. (b) Typical images of H9c2 cells subject to amifostine pretreatment. 1: control; 2: 200 μM TBHP; 3: 5 μM amifostine; 4: 10 μM amifostine; 5: 20 μM amifostine. (c) Representative bands of cleaved SOD2 and quantitative analysis results. Values are expressed as mean ± SEM. versus control group; versus 200 μM TBHP group; per group.

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