Resveratrol Ameliorates Mitochondrial Elongation via Drp1/Parkin/PINK1 Signaling in Senescent-Like Cardiomyocytes

Figure 1

Drp1 mediated mitochondrial elongation induced by D-galactose. (a) H9c2 cardiomyocytes were treated with D-galactose (D-Gal; 0, 10, 20, and 40 g/l) and Mdivi-1 (40 μM) for 48 hours, respectively. Mitochondrial morphology was detected using a confocal microscope by MitoView Red staining (upper panels); objective magnification, 63x; white scale bar represents 20 μm. Magnified photographs showed a detail view of the area indicated in the upper panels. (b) Drp1 expressions in H9c2 cells treated with D-galactose and Mdivi-1 were detected by immunoblot analysis. Actin was represented as the loading control. The relative values were normalized to actin. Data () were shown as the mean ± SEM (). (c) Mfn1, Mfn2, and OPA1 expressions were detected by immunoblot analysis. Actin was represented as the loading control. The relative values were normalized to actin. Data () were shown as the mean ± SEM. NS: no significance ().

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