Research Article
Comparative Analysis of AGE and RAGE Levels in Human Somatic and Embryonic Stem Cells under H2O2-Induced Noncytotoxic Oxidative Stress Conditions
Figure 3
Differentiation of functional hESC-derived CMs. (a) Gene expression patterns during HUES7 cardiomyogenesis. Real-time PCR studies showed that cardiomyocyte differentiation was characterized by a continuous decrease in the expression of pluripotent markers (OCT4 and NANOG) coupled with an initial increase in mesoderm and cardiomesoderm markers (Brachyury and MESP1). This was followed by the expression of a secondary heart field progenitor marker (ISL1), by cardiac-related transcription factor (nKx2-5, MEF2-C, and GATA4), and finally, by a cardiac-specific structural gene (cTNN1) and ionic channels (CACNA-1C). Three qRT-PCR analyses were conducted with each of the 3 independent replicates. Bars depicted the mean ± SD of the relative gene expression at each time point as normalized by baseline values. (b, c) Feeder-free HUES7 cells grown on matrigel. (d, e) Embryoid bodies aggregated from HUES7 cells after 7 days of cardiac differentiation. (f, g). Representation of two areas of contraction on differentiated cardiomyocytes after 25 days of cardiac differentiation. White scale bar = 50 μm.
| (a) |