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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 4256519, 12 pages
https://doi.org/10.1155/2017/4256519
Research Article

Potassium Ascorbate with Ribose: Promising Therapeutic Approach for Melanoma Treatment

1Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy
2Plants for Human Health Institute, Department of Animal Sciences, North Carolina State University, Kannapolis, NC, USA
3Department of Medicine, Surgery and Neurosciences, University of Siena, Siena, Italy
4Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Siena, Italy
5Department of Molecular and Developmental Medicine, University of Siena, Siena, Italy
6Department of Life Sciences, University of Siena, Siena, Italy

Correspondence should be addressed to Giuseppe Valacchi; ti.efinu@ihccalav.eppesuig

Received 20 May 2017; Accepted 10 July 2017; Published 24 September 2017

Academic Editor: Jean-Claude Lavoie

Copyright © 2017 Carlotta Cavicchio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

While surgery is the definitive treatment for early-stage melanoma, the current therapies against advanced melanoma do not yet provide an effective, long-lasting control of the lesions and a satisfactory impact on patient survival. Thus, research is also focused on novel treatments that could potentiate the current therapies. In the present study, we evaluated the effect of potassium ascorbate with ribose (PAR) treatment on the human melanoma cell line, A375, in 2D and 3D models. In the 2D model, in line with the current literature, the pharmacological treatment with PAR decreased cell proliferation and viability. In addition, an increase in Connexin 43 mRNA and protein was observed. This novel finding was confirmed in PAR-treated melanoma cells cultured in 3D, where an increase in functional gap junctions and a higher spheroid compactness were observed. Moreover, in the 3D model, a remarkable decrease in the size and volume of spheroids was observed, further supporting the treatment efficacy observed in the 2D model. In conclusion, our results suggest that PAR could be used as a safe adjuvant approach in support to conventional therapies for the treatment of melanoma.