Pentaerythritol Tetranitrate In Vivo Treatment Improves Oxidative Stress and Vascular Dysfunction by Suppression of Endothelin-1 Signaling in Monocrotaline-Induced Pulmonary Hypertension

Figure 4

Effects of PETN therapy on endothelial function, oxidative stress, and protein tyrosine nitration of aorta and pulmonary artery in monocrotaline-treated rats (MCT40 4 weeks). (a, b) Endothelium-dependent (ACh) relaxation was determined by isometric tension studies in rat aortic ring segments and pulmonary ring segments. (c) DHE (1 µM) oxidative fluorescence microtopography was used to assess vascular oxidative stress. (d) Levels of 3-NT positive proteins in lung tissue were assessed by dot blot analysis and specific antibodies. Representative blots are shown below the densitometric quantification. A total number of 6–22 aortic (a) and 6–17 pulmonary tissue (b–d) ring segments from male rats were used. versus control and versus MCT40.

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