Activation of the Nrf2-Keap 1 Pathway in Short-Term Iodide Excess in Thyroid in Rats

Figure 2

Effects of the iodide intake (NI, 10 HI, and 100 HI) on the changes of mitochondrial superoxide production on Days 7, 14, and 28. (a) There was a significant increase in the mitochondrial superoxide production in the 100 HI group on Days 7, 14, and 28 (). The difference was more significant on Day 28 when compared to Day 7 or Day 14. (b) Histogram analysis was performed on the mean fluorescence intensity of MitoSOX Red. All data is presented as the mean ± SD ( for each group). Statistical analyses were performed by one-way analysis of variance (ANOVA) with the Least Significant Difference (LSD) test. versus the NI group on Days 7, 14, and 28, respectively. versus the 10 HI group on Days 7, 14, and 28, respectively. versus the Day 7 group in the 100 HI group. versus the Day 14 in the 100 HI group. Experiments were repeated 3 times with similar results. (c) There was a significant increase in the mitochondrial superoxide production in the 100 HI group on Day 28 and was observed by confocal microscopy. Scale bar: 20 µm.

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