Hypoxia Downregulates MAPK/ERK but Not STAT3 Signaling in ROS-Dependent and HIF-1-Independent Manners in Mouse Embryonic Stem Cells

Figure 6

HPLC determination of specific 2-OH-E(+) (a) and nonspecific product E(+) (b) of HE oxidation in cells cultivated in normoxia or hypoxia or supplemented with GSH. Data are presented as mean + SEM from at least three independent experiments. Statistical significance was determined by one-way analysis of variance ANOVA and post hoc Bonferroni’s multiple comparison test (). Effect of GSH and β-ME supplementation on phosphorylation of MAPK/ERK, Akt, and STAT3 (c, d). Total level of β-actin was used as a loading control.

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