Hypoxia Downregulates MAPK/ERK but Not STAT3 Signaling in ROS-Dependent and HIF-1-Independent Manners in Mouse Embryonic Stem Cells

Figure 7

Relative expression of OCT4 (a), NANOG (b), ZFP42 (c), TNAP (d), and FGF5 (e) in wild-type ES cells determined by qRT-PCR. Data are presented as mean + SEM from at least three independent experiments. Statistical significance was determined by T test (). Protein level of Oct4 and Nanog in wild-type ES cells cultivated in normoxia or hypoxia as determined by western blot (f). Total level of β-actin was used as a loading control. Determination of alkaline phosphatase activity in wild-type ES cells cultivated in normoxia or hypoxia (g). Data are presented as mean + SEM from at least three independent experiments. Statistical significance was determined by T test ().

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