Hypoxia Downregulates MAPK/ERK but Not STAT3 Signaling in ROS-Dependent and HIF-1-Independent Manners in Mouse Embryonic Stem Cells

Table 1

Primers, appropriate annealing temperatures, and PCR product lengths.

Transcript	Forward 5′→3′	Reverse 5′→3′	Annealing (°C)	Product (bp)
DUSP1	tctcagccaattgtgctaac	tgatggagtctatgaagtcaa	56	126
DUSP5	cgcctacagaccagcctatg	ccttcttccctgacacagtcaat	61	264
DUSP6	acctggaaggtggcttcagt	tccgttgcactattggggtc	62	187
FGF5	accggtgaaaccaaaggtg	gcgaaacttcagtctgtacttcact	57	76
GDF15	ccgagaggactcgaactcag	ggttgacgcggagtagcag	60	104
NANOG	aagcagaagatgcggactgt	gtgctgagcccttctgaatc	59	232
OCT4	cgttctctttggaaaggtgttc	gaaccatactcgaaccacatcc	59	319
PGK1	ggtgttgccaaaatgtcgcttt	cagccttgatcctttggttgtt	58	139
PP2A	gtggatgggcagatcttctgt	gcagcttggttaccacaacg	60	347
TBP	gcacaggagccaagagtgaag	acacgtggatagggaaggca	60	397
TNAP	aggcaggattgaccacgg	tgtagttctgctcatgga	59	440
VEGF	agaaggagagcagaagtccc	gatccgcatgatctgcatgg	62	235
ZFP42	gcacacagaagaaagcagga	cactgatccgcaaacacct	57	94
β-actin	gatcaagatcattgctcctcct	taaaacgcagctcagtaacag	59	177