Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants

Figure 2

Cell death. (a) Representative bright field and PI overlay images of control (upper row) and plasma-treated (120 s, lower row) cells at different time points following exposure. (b) Representative dot plot of control cell area versus PI intensity per cell. (c) Representative dot plot of plasma-treated (120 s) cells and their area versus PI intensity per cell. Image quantification and normalization of PI⁺ cells of all cells per field of view. Data are presented as mean ± S.E. of nine replicates. One representative of three independent experiments is shown. Statistical analysis was performed using t-test.

(a)

(b)

(c)

(d)