Tanshinone IIA Inhibits Glutamate-Induced Oxidative Toxicity through Prevention of Mitochondrial Dysfunction and Suppression of MAPK Activation in SH-SY5Y Human Neuroblastoma Cells

Figure 1

Effect of tanshinone IIA on glutamate cytotoxicity in SH-SY5Y cells. (a) Relative viability of SH-SY5Y cells treated with tanshinone IIA at the indicated concentrations at 37°C for 24 h. (b) Relative viability of SH-SY5Y cells pretreated with tanshinone IIA at the indicated concentrations for 24 h and then exposed to 10 mM glutamate for another 24 h. (c) Relative level of LDH release of the SH-SY5Y cells treated as in (b). All data are normalized to the cells without tanshinone IIA treatment and glutamate exposure and presented as mean ± SEM of three independent experiments. Tan IIA: tanshinone IIA; Glu: glutamate. compared to the cells without tanshinone IIA treatment (a) and the cells exposed to glutamate alone ((b) and (c)).

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