Tanshinone IIA Inhibits Glutamate-Induced Oxidative Toxicity through Prevention of Mitochondrial Dysfunction and Suppression of MAPK Activation in SH-SY5Y Human Neuroblastoma Cells

Figure 2

Effect of tanshinone IIA on mitochondrial membrane potential, protein carbonyl content, and ATP content in glutamate-exposed SH-SY5Y cells. The cells were pretreated with tanshinone IIA at the indicated concentrations and then exposed to 10 mM glutamate. (a) Mitochondrial membrane potential was determined using the JC-1 fluorescence probe and presented as the ratio of red to green fluorescence. (b) Mitochondrial protein carbonyl content was determined using a colorimetric assay. (c) Cellular ATP content was measured using a chemiluminescence method. The results are shown as mean ± SEM of three independent experiments. Tan IIA: tanshinone IIA; Glu: glutamate. compared to the cells exposed to glutamate alone.

(a)

(b)

(c)